ABSTRACT
Objective To investigate the change of the percentage of Th17 cells and cytokine among hepatocellular carcinoma (HCC) tissues and para-carcinoma tissue and the liver tissue of hepatic hemangio-ma, and to explore its clinical significance. Methods To choose 26 of hepatocellular carcinoma patients between November 2014 and December 2016 and also include 11 cases of hepatic hemangioma as control group. The percentage of Th17cells in HCC tissue and para-carcinoma tissue and the liver tissue of hepatic hemangioma was evaluated by flow cytometric; The levels of IL-6 and IL-17 in HCC tissues, para-carcinoma tissue and the liver tissue of hepatic hemangioma were detected by RT-PCR. Results The ratio of Th17 cells to CD4 cells in peripheral blood of HCC was significantly higher than that in control group (4. 07% ± 0. 68% vs. 1. 39% ± 0. 41% ), P＜0. 05. The ratio of Th17 cells to CD4 cells in patients with hepatocellular carcinoma was significantly higher than that in paracancerous tissues and liver tissues of hepatic hemangioma (4. 76% ± 0. 75% vs. 2. 30% ± 0. 26% vs. 0. 77% ± 0. 31% , P＜0. 05). Multivariate linear stepwise regression analysis of the changes of Th17 cells in patients with HCC and their correlation with clinical parameters showed that Th17 lymphocytes in peripheral blood of patients with HCC were closely related to TNM staging(YTh17 =2. 647 +0. 687TNM. It’s indicated that the level of IL-6 and IL-17 in liver cancer tissues was significantly higher than that of the liver hemangioma in the control group through ELISA ( P＜0. 05). The proportion of peripheral blood Th17 cells to CD4 cells is different in different TNM stages(stage I＜stage II ＜stage III ～IV, the difference was statistically significant. RT-PCR analysis showed that the expression of IL-17, IL-6mRNA in hepatocellular carcinoma was significantly higher than that in adjacent tissues and hepatic hemangioma liver tissue (all P＜0. 05). Conclusion The increased proportion of Th17 cells and the increased levels of cytokines IL-6 and IL-17 may be closely correlated with occurrence and development of hepatocellular carcinoma. It can be an important target of anti-tumor therapy.
ABSTRACT
Objective To investigate the expression of serum miR-140-3p in children with autism spectrum disorder (ASD) and its correlation with interleukin-4 ( IL-4),interleukin-8 (IL-8) and interleu-kin-17A (IL-17A),and provide evidence for the diagnosis and treatment of ASD. Methods Totally 172 ca-ses of ASD children admitted to Anning Hospital of Hainan Province from January 2015 to June 2018 were selected as case group,80 cases of healthy subjects as control group. ASD children were divided into mild-moderate group (n=116) and severe group (n=56) by ASD Behavior Rating Scale and Child Autism Rating Scale. Real-time fluorescence quantitative polymerase chain reaction ( RT-PCR) and enzyme-linked immu-nosorbent assay ( ELISA) were used to detect the changes of serum levels of miR-140-3p,IL-4,IL-8 and IL-17A in each group. ROC curve was used to analyze the diagnostic value of miR-140-3p,IL-4,IL-8 and IL-17A for ASD. Pearson correlation was used to analyze the correlation between serum miR-140-3p and IL-4,IL-8 and IL-17A in children with ASD. Results The serum levels of miR-140-3p ( 0. 86 ± 0. 17 vs 0. 15±0. 03),IL-4(48. 65±13. 82)pg/ml vs (31. 42±7. 50)pg/ml),IL-8((7. 14±2. 05) pg/ml vs (2. 30± 0. 74)pg/ml) and IL-17A((112. 35±51. 64)pg/ml vs (58. 20±26. 40)pg/ml) in the case group were sig-nificantly higher than those in the control group ( P<0. 05). Serum levels of miR-140-3p( 1. 08 ± 0. 24 vs 0. 71±0. 12),IL-4((53. 28±16. 30)pg/ml vs (43. 70±13. 52)pg/ml),IL-8((9. 42±2. 85)pg/ml vs (5. 63 ±1. 48)pg/ml) and IL-17A((138. 40±65. 72)pg/ml vs (96. 74±40. 83) pg/ml) in severe group were sig-nificantly higher than those in mild-moderate group(P<0. 05). The AUC (95%CI) of serum miR-140-3p for ASD diagnosis was 0. 827 (0. 773-0. 886),which were significantly higher than that of IL-4 (0. 719 (0. 651-0. 764)pg/ml),IL-8 (0. 683 (0. 625-0. 743)pg/ml) and IL-17A(0. 745 (0. 683-0. 817)pg/ml). The best cut-off value was 0. 75,and the sensitivity and specificity were 84. 8% and 78. 2%,respectively. The best cut-off value of serum miR-140-3p for diagnosing ASD was 0. 75, and its sensitivity and specificity were 84. 8% and 78. 2%,respectively. The correlation analysis showed that serum miR-140-3p was positively cor-related with IL-4,IL-8 and IL-17A in children with ASD (r=0. 681,r=0. 624,r=0. 775,all P<0. 01). Con-clusion Serum levels of miR-140-3p was significantly higher in ASD children, correlated with levels of IL-4,IL-8 and IL-17A,and miR-140-3p was a potential biomarkers for ASD diagnosis.